By virtue of the authority of the Secretary of Health under Executive Order No. 119, dated January 30, 1987, to promulgate rules and regulations necessary to carry out the Department of Health objectives, policies, plans, programs and projects, pursuant to Section 3, General Provisions of Republic Act No. 8250, otherwise known as General Appropriations Act of CY 1997, the following fees shall be charged for certain laboratory examinations performed in the Bureau of Research and Laboratories, this Department:

	Laboratory Examination			Fee
A.	BACTERIOLOGY
1.	Direct Smear for Acid Fast Bacilli (AFB)			60.00
2.	Gram Stain			60.00
3.	Flourescent Acid Fast Microscopy (FAM) test for AFB			60.00
4.	Culture for Mycobacterium Tuberculosis (MTB) (Conventional Method)			415.00
5.	Sensitivity Test for MTB (Conventional)			250.00
6.	Culture for Enteric Organism
	a. Salmonella/Shigella b. Vibrio c. E. coli d. Aeromonas/Plesiomonas e. Campylobacter f. Yersinia			550.00 550.00 550.00 550.00 550.00 550.00
7.	Sensitivity Test for the above Enteric Organism			150.00
8.	Blood Culture with sensitivity for S. Typhi			415.00
9.	Culture & sensitivity for N. Gonorrhea			310.00
10.	Culture & sensitivity for H. Influenza			550.00
11.	Culture & sensitivity for Streptococci			560.00
12.	Culture & sensitivity of Staphylococci			380.00
13.	Culture & sensitivity for N. Meningitis			470.00
14.	Other microorganisms from other body fluids			540.00
B.	ENVIRONMENTAL HEALTH - WATER ANALYSIS
1.	Bacteriological Examination of Water
	1.a Total Coliform MPN Tests 1.b IMVic Test 1.c Heterotrophic Plate Count 1.d Fecal Coliform Test (E.C. Test)			90.00 110.00 35.00 70.00
2.	Physical/Chemical Exam of Water			1,050.00
			(20 Parameters) Odor Color Turbidity pH Residual Acidity
	Alkalinity
			Total Chloride
	Total Hardness
			Total Iron
	Total Solids
			Total Dissolved Solids Fluoride Nitrite Chlorine Calcium Magnesium Manganese Sulfate Total Suspended Solids Silica
C.	IMMUNOLOGY
1.	Serologic Test for Syphilis
	1.1 Venereal Disease Research Laboratory(VDRL) 1.2 Rapid Plasma Reagin (RPR) 1.3 Treponema Pallidum Hemagglutination(TPHA) 1.4 Flourescent Treponema- Antibody Absorption (FTA-ABS)			85.00 85.00 150.00 350.00
2.	Hepatitis A Virus (HAV-Igm)			275.00
3.	Hepatitis B
	3.1 HBsAg (Rapid Plasma Hemagglutination - RPHA) 3.2 HBsAg (Immunochromatography Assay) 3.3 HBsAg (Enzyme Immuno Asay - EIA) 3.4 HBsAg (Confirmatory) 3.5 Anti-HBc 3.6 Anti- HBs 3.7 Hbe/Anti-Hbe			180.00 110.00 160.00 600.00 200.00 200.00 350.00
4.	Hepatitis C
	4.1 HCV Antibody (Particle Agglutination - PA) 4.2 HCV Antibody (EIA) 4.3 Hepatitis C Virus (HCV - Confirmatory)			260.00 20.00 2,695.00
5.	HIV Antibody
	5.1 HIV-1/2 (Dot Immunoassay) 5.2 HIV-1 Antibody (PA) 5.3 HIV-1/2 Antibody (PA) 5.4 HIV Antibody (EIA) 5.5 Western Blot (HIV) 5.6 Line Immunoassay (LIA-HIV)			150.00 170.00 220.00 240.00 1,300.00 2,375.00
D.	IMMUNOHEMATOLOGY
1.	Referral Laboratory Services
	a. ABO Sub-Grouping b. Confirmation of weak A or B c. Weak D Test d. Red Cell Phenotyping			385.00 2,155.00 315.00
			d.1 Rh Phenotyping d.2 Full Red Cell Phenotyping	215.00 1,390.00
	e. Antibody Screening Test
			e.1 Saline Technique e.2 Low-ionic Strength Solution Technique (LISS) e.3 Albumin Technique e.4 Enzyme	130.00 145.00 145.00 210.00
	f. Antibody Identification Test
			f.1 Saline f.2 LISS f.3 Albumin f.4 Enzyme	1,635.00 2,130.00 2,100.00 1,800.00
	g. Compatibility Testing
			g.1 Bovine Albumin g.2 LISS g.3 Enzyme	405.00 410.00 430.00
2.	Routine Services
	a. Blood Grouping			100.00
			a.1 ABO grouping a.2 Rh Typing	60.00 40.00
	b. Direct Antiglobulin Test c. Indirect Antiglobulin Test			150.00 120.00
E.	HEMATOLOGY
1.	Referral Laboratory Services
	a. Cytochemistry (Confirmation of Leukemia Limeage)
			a.1 Wright ™s Giemsa a.2 Periodic Acid Schiff (PAS) a.3 Sudan Black B (SBB) a.4 Myeloperoxidase (MPO) a.5 Alpha Napthyl Acid Esterase (ANAE) a.6 Alpha Napthyl Butyrate Esterase (ANBE) a.7 Napthol AS-D Chloroacetate (Exterase (NASDCAE) a.8 Leukocyte Alkaline Phosphatase (LAP) a.9 Acid Phosphatase (ACP)	110.00 150.00 155.00 160.00 155.00 165.00 155.00 155.00 180.00
	b. Leukemia - Immunophenotyping (8 anti- body staining reagent panel) c. Lymphoma Screening ( 6 antibody screening panel)			2,100.00 2,100.00
	d. Hemolytic anemia
			G-6-PD Assay Osmotic Fragility Test Acid serum Test (Ham ™s test) Staining for Heinz bodies and iron Sugar Water Test	450.00 185.00 170.00 115.00 105.00
2.	Routine Services
	a. CBC with platelet (Automated) b. Erythrocyte Sedimentation Rate (ESR) (Winthrobe) c. Reticulocyte Count (NMB supravital Staining ) d. Prothrombin Time (Automated ) e. Activated Prothrombin Time (Automated) f. Clotting Time (Manual; Lee & White) g. Bleeding Time (Disposable Bleeding Time Device) h. L.E. Prep. (manual)			140.00 25.00 60.00 215.00 260.00 40.00 200.00 135.00
F.	ANATOMIC PATHOLOGY SECTION
1.	Exfoliative Cytology
	1.a Routine Papanicolaou Staining (PAPs) 1.b Cell block 1.c Complete Examination for Papanicolaou/ Cell Block 1.d Laboratory Processing :			60.00 70.00 130.00
		PAP ™s (1-2 slides) Cell Block (1-2 slides) Pap ™s/Cell Block (3-4 slides)		50.00 60.00 120.00
2.	Histopathology
	2.a Complete examination for 1 and/or 2 small specimens 2.b Complete examination for 3 small and 1 large specimens 2.c Complete examination for 2 large specimens 2.d Complete examination for radical specimens			100.00 120.00 160.00 220.00
3.	Laboratory Processing
	3.a 1 and/or 2 small specimens 3.b 3 small or 1 large specimens 3.c 2 large specimens 3.d Radical specimens			90.00 110.00 140.00 170.00
4.	Additional Slides (per slide)			30.00
G.	PARASITOLOGY AND MEDICAL ENTOMOLOGY
1.	Routine Laboratory Service Capability
	1.a Direct Fecal Smear 1.b Filarial smear (Giemsa stain) 1.c Malarial smear (Giemsa stain) 1.d Scotch tape method 1.e Test for occult blood			30.00 50.00 50.00 50.00 30.00
2.	Reference Laboratory Service capability
	2.a Concentration method for stool 2.b Staining for amoeba			50.00 50.00
			2.b.1 Iron Hematoxylin 2.b.2 Trichrome stain 2.b.3 Chlorazol Black E stain
3.	Staining of adult worms Sechon Acetic Carmine Method			50.00
4.	Identification of Cryptosporidium by staining of modified Acid Fast stain			50.00
5.	Nucleopore membrane Technique for Filaria(Conc. method)			75.00
6.	Concentration method for Pulmonary Flukes			50.00
7.	Circum-Oval Precipitin Test for Schistosoma (COOPT)			50.00
H.	MEDICAL MYCOLOGY
1.	KOH Smear			60.00
2.	Mycologic culture			100.00
I.	BIOCHEMISTRY
1.	Routine Clinical Chemistry
	a. Glucose b. Uric Acid c. Cholesterol d. Creatinine e. Blood Urea Nitrogen (BUN) f. Alanine Aminotransferase (ALT) g. Aspartate Aminotransferase (AST) h. Total Protein i. Albumin j. Alkaline Phos k. Total Bilirubin			60.00 70.00 60.00 60.00 60.00 65.00 65.00 60.00 60.00 75.00 70.00
2.	Special Test/ Special Program [Program Against Micronutrient Malnutrition (PAMM)]
	a. Urine Iodine b. Thyroid Stimulating Hormone (TSH) (Blood Spot)			80.00 40.00

The rate of laboratory fees herein promulgated is within the range of usual fees prevailing at the time, and in the different public health laboratories, taking into consideration the cost of reagents, supplies and materials, and qualify control of various laboratory procedures.

This Order supersedes all previous issuances inconsistent thereof and shall take effect after fifteen (15) days following its publication in the newspaper of general circulation.

Adopted: 23 July 1997

(SGD.) CARMENCITA NORIEGA-REODICA
M.D., M.P.H. CESO III
Secretary of Health